Confocal Microscopy: Principles, Techniques, and Applications
Confocal Microscopy: Techniques and Applications
Samples can be fixed or unfixed.
Results can be correlated with DIC (Nomarski) imaging.
Oriented to:
- Study of thick samples in fluorescence (fixed or unfixed).
- Study of samples marked with 1, 2, 3, or 4 fluorescent molecules during in vivo experiments in cultured cells with variable time lengths.
- Three-dimensional reconstruction of thick specimens studied in fluorescence.
- In vivo experiments in cultured cells requiring complex handling areas for stimulation
Understanding Raw Materials, Manufactured Goods, and Material Properties
Raw Materials and Manufactured Goods
Raw materials are substances extracted directly from natural sources, whether of animal, vegetable, or mineral origin. Manufactured goods are any objects created by humans to satisfy their needs and improve their standard of living.
Types of Materials
Wood
Tree trunks (fir and pine) used for furniture, floors, and paper.
Metals
Ferrous metals: Contain iron (e.g., steel).
Non-ferrous metals: Do not contain iron (e.g., copper, bronze, tin, zinc, and aluminum). Used for
Read MorePetroleum and Natural Gas: Processing and Applications
Transformation into Products Suitable for Consumption
In refineries, a series of processes are carried out that aim to separate the different components or fractions of petroleum.
Fractional Distillation
Fractional distillation involves heating the oil until it evaporates and then cooling it to obtain different fractions based on their boiling points. This is done in distillation towers (working at atmospheric pressure) or fractionation columns (under vacuum). The process begins by heating the oil
Read MoreSmart Home Automation Systems: Wiring and Pre-Installation
Wired systems use physical wire connections to capture data and control elements. This method is traditionally used in conventional power plants.
In programmed systems, signal reception items, such as performance metrics, are connected to an electronic device. This device is programmed to define the operation of the circuit.
A device can be considered part of a home automation system if it can send or receive information from other system elements.
Circuits in a Home Automation Installation
Power Supply
Read MoreUnderstanding S-Expression Operators: Less, Eq, If0, App, and Rec
S-Expression Operators: Less, Eq, If0, App, and Rec
- Less refers to the less than operator (<). This checks both the left and right sides of the S-expression to ensure they are both `numType`. It then returns a `boolType`. In the assignment, it was unclear if this should also check if both sides were `boolType`.
[eq (l r)(if (and (numType? (get-type l tenv))(numType? (get-type r tenv)))(boolType)(error 'get-type "You need to match the types for the equality check operation"))]
- Eq refers to the
Understanding Car Suspension Systems: Types & Components
Conventional Suspension Systems
Mission: To minimize the impact of terrain irregularities on the vehicle body.
Types of Suspension:
- Independent: When one wheel moves, it does not affect the other.
- Dependent (Rigid): Movement of one wheel directly affects the other.
Components:
- Elastic Elements
- Shock Absorbers
- Linkages
- Stabilizer Bar
- Suspension Arms
Elastic Elements:
Springs: Helical coiled steel rods. (Variations include springs with closer turns and truncated springs.)
Features:
- Wire diameter: Thickness of the