Biochemical Analysis: Lipids, Nucleic Acids
Isolation and Purification of Lecithin from Egg Yolk
Egg yolks are a rich and convenient source of lecithin. They contain water, fats, proteins, phospholipids, cholesterol, pigments, and vitamins. Using acetone extraction, most triacylglycerols, pigments, and vitamins can be removed, leaving mainly protein and phospholipid residue. Further extraction with chloroform-methanol solvents can remove the protein and provide a crude egg lecithin solution. Finally, lecithin can be separated from other contaminants
Read MoreAssisted Reproduction, Cloning, and Bioethics: An Overview
Assisted Reproductive Procedures
Assisted reproductive procedures include two main techniques:
- Artificial Insemination (AI): This involves introducing semen into the uterus through a cannula. The semen can be obtained from the male partner or from an anonymous donor through a sperm bank.
- In Vitro Fertilization (IVF): This involves achieving fertilization in a laboratory setting and then implanting the embryo into the mother’s uterus. Implementation consists of implanting the embryo in the wall of the
Lipids: Essential Biomolecules and Their Roles
Lipids: Structure and Classification
Lipids are a structurally diverse group of biomolecules. The only common characteristic is that a significant part, or all, of their molecule is insoluble in water and soluble in organic solvents (alcohol, acetone, ether, chloroform, etc.). Unlike proteins, nucleic acids, and polysaccharides, lipids do not form large polymers.
Classification of Lipids
Fatty Acids and Derivatives
Fatty acids are long-chain carboxylic acids. The hydrocarbon chain can be saturated or
Read MorePharmacodynamics: How Drugs Affect the Body
Pharmacodynamics
Pharmacodynamics is the study of how drugs affect the body. It explores drug interactions with biological systems and how these result in therapeutic or adverse effects. Understanding pharmacodynamics is crucial for comprehending both desired and undesired drug effects.
1. Mechanism of Drug Action
Drugs interact with specific molecules (often proteins like receptors, enzymes, or ion channels) to alter cellular functions, producing therapeutic or pharmacological effects.
Receptors
Receptors,
Read MoreGMOs: Structure, Transgenic Organisms, Labeling, and Applications
DNA Structure and Transgenic Organisms
DNA Structure
The structure of DNA is a double helix of two antiparallel strands. It is composed of nucleotides (deoxyribose, phosphate, and nitrogenous bases).
Transgenic Organisms
A transgenic organism is a living being into whose genome the genetic material of another living organism has been introduced. These organisms are artificially created by manipulating their genes. Eukaryotic organisms, such as plants or animals, that have been modified by genetic engineering
Read MoreMicrobiology Lab Equipment and Pinus Needle Leaf Anatomy
Essential Equipment in Microbiology Labs
Microbiology laboratories rely on specialized equipment to ensure sterile conditions and accurate results. Here’s a look at some key instruments:
- Laminar Air Flow (LAF): Laminar airflow cabinets are crucial for creating a sterile workspace in microbiology labs. They are essential when handling sensitive samples or conducting experiments requiring aseptic techniques. LAF cabinets minimize airborne contaminants, reducing the risk of introducing unwanted microbes